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cymbopogon citratus essential oil

Biological Definition Cymbopogon Citratus Leaf Oil is an essential oil obtained from the leaves of the Lemon Grass, Cymbopogon citratus, Poaceae. Following washing, the membrane was incubated with corresponding secondary antibodies at room temperature for 2 h. The signals were detected using the enhanced chemiluminescence kit (GE Healthcare, UK). How to use essential oils safely - general safety guidelines. doi:10.3390/medicines3040025, Han X, Parker T. Lemongrass () essential oil demonstrated anti-inflammatory effect in pre-inflamed human dermal fibroblasts. The samples were taxonomically identified by Dr. Nguyen, The Cuong, Institute of Ecology and Biological Resources (VAST), and voucher specimens were deposited in the Advanced Center for Bio-organic Chemistry. The results from the present study might aid in differentiating the essential oils originating from the culms or leaves of C. citratus. Camptothecin was used as a positive control. This oil can be used in food preservation systems to inhibit the growth of moulds and retard subsequent mycotoxin production. Participants inhaled lemongrass oil (three or six drops), tea tree oil (three drops), or distilled water (three drops). J Clin Diagn Res. View abstract. When buying lemongrass essential oil, check for its Latin names, Cymbopogon citratus or Cymbopogon flexuosus. The Kim Duc culm (KDC) essential oil exerted the strongest cytotoxic effects against H1650 cells, with an IC50 value of 4.86 ± 0.29 μg/mL. J Adv Pharm Technol Res. 2015;22(4):226-9. doi:10.1159/000432407, Madeira P, Carvalho L, Paschoal M, et al. Researchers gave subjects with dandruff a hair tonic containing either lemongrass essential oil (Cymbopogon flexuosus) or a placebo twice a day. The western blot analysis indicated that HLL induced the apoptotic effect by altering the regulating proteins of the apoptosis process such as caspase-3, Bcl-2, and Bax. citratus Cymbopogon giganteus Essential oils Chemical composition Antimicrobial Combination a b s t r a c t As part of ongoingresearchon the chemical composition and the antimicrobial properties of Burkinabe plantsessentialoilsaloneand in combination, essentialoils(EOs)from leavesofCymbopogoncitratusand Cymbopogongiganteusfrom Burkina Fasowere analyzed by GC FID … We are committed to sharing findings related to COVID-19 as quickly as possible. 2011;2011:467134. doi:10.1093/ecam/nep213. Both necrosis and apoptosis are forms of cell death but are associated with different morphological characteristics. MassFinder 4.0 software was used for data analysis. These results show that the essential oil of C. citratus has antifungal activities against fungi that are the producers of poisonous mycotoxins found in foods. Phytomedicine. A549 cells were treated with different concentrations (0, 1, and 10, Advanced Center for Bio-Organic Chemistry, Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, Viettri University of Industry, Tien Kien, Lam Thao, Phu Tho, Vietnam, University of Science and Technology of Hanoi, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, Center for Research and Technology Transfer, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, V. S. Gadepalli, S. P. Deb, S. Deb, and R. R. Rao, “Lung cancer stem cells, p53 mutations and MDM2,”, P. Zarogoulidis, K. tsirgogianni, K. Darwiche et al., “EGFR-TKIs in adjuvant treatment of lung cancer: to give or not to give?”, K. Bencardino, M. Manzoni, S. Delfanti, A. Riccardi, M. Danova, and G. R. Corazza, “Epidermal growth factor receptor tyrosine kinase inhibitors for the treatment of non-small-cell lung cancer: results and open issues,”, A. K. Handique and H. B. Singh, “Antifungal action of lemongrass oil on some soil-borne plant pathogens,”, Z. T. Pinto, F. F. Sánchez, A. R. d. Santos et al., “Chemical composition and insecticidal activity of, K. Koba, K. Sanda, C. Guyon, C. Raynaud, J.-P. Chaumont, and L. Nicod, “In vitro cytotoxic activity of, S. Kpoviessi, J. Bero, P. Agbani et al., “Chemical composition, cytotoxicity and in vitro antitrypanosomal and antiplasmodial activity of the essential oils of four, M. Saleem, N. Afza, M. A. Anwar, S. M. A. Hai, and M. S. Ali, “A comparative study of essential oils of, P. Vyshali, K. J. T. Saraswathi, and G. R. Mallavarapu, “Chemical composition of the essential oils of, B. K. Matouschek and B. E. Stahl, “Phytochemical study of non volatile substances from, K. H. Miean and S. Mohamed, “Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants,”, H. T. K. Van, N. M. Quy, D. T. V. Ha et al., “Chemical composition and cytotoxic activity of the essential oils of, M. M. Blanco, C. A. R. A. Costa, A. O. Freire, J. G. Santos Jr., and M. Costa, “Neurobehavioral effect of essential oil of, G. F. Santoro, M. G. Cardoso, L. G. L. Guimarães, J. M. Freire, and M. J. Soares, “Anti-proliferative effect of the essential oil of, R. Thangam, M. Sathuvan, A. Poongodi et al., “Activation of intrinsic apoptotic signaling pathway in cancer cells by, N. Dudai, Y. Weinstein, M. Krup, T. Rabinski, and R. Ofir, “Citral is a new inducer of caspase-3 in tumor cell lines,”, L. Quassinti, F. Maggi, L. Barboni et al., “Wild celery (, N. Girola, C. R. Figueiredo, C. F. Farias et al., “Camphene isolated from essential oil of, K. Spyridopoulou, A. Tiptiri-Kourpeti, E. Lampri et al., “Dietary mastic oil extracted from, M. Cho, I. Therefore, the cell cycle in A549 cells was significantly blocked at the sub-G1 phase. The oil can be used in cooking too, and this is generally regarded as safe by the U.S. Food and Drug Administration (FDA). The membrane was blocked with 5% nonfat skim milk at room temperature for 1 h, followed by washing with PBS containing 0.1% Tween-20. The cellular proliferation of this panel of four NSCLC cell lines was examined by MTT assay over a 48 h period in the presence of increasing concentrations of various C. citratus essential oils. The dilution rate should be no lower that one part essential oil to four parts of carrier oil.. Investigation of the mechanisms underlying the gastroprotective effect of cymbopogon citratus essential oil. In addition, the cytotoxic effects on different subtypes of NSCLC cell lines and the anticancer mechanism of lemongrass essential oils were investigated preliminarily. Lemongrass (Cympopogon citratus L.) is a plant in the grass family that contains 1 to 2% essential oil on a dry basis with widely variation of the chemical composition as a function of genetic diversity, habitat and agronomic treatment of the culture (Carlson, Machado, Spricigo, Periera, & … In previous studies, the essential oil of C. citratus was shown to exert various pharmacological activities, including antimicrobial [6], insecticidal [7], and cytotoxic activities [8, 9]. In particular, camphene, valerianol, and epi-α-muurolol can be used to differentiate essential oils originating from leaves versus culms. A 2015 study found lemongrass essential oil may help fight dandruff. Currently, EGFR-TKIs significantly extend the progression-free survival of patients with NSCLC. Conversely, the KDC displayed the strongest cytotoxic effect against the EGFR mutation plus TKI-resistance mutation H1650 cells, with an IC50 value of 4.86 μg/mL, and HCL presented the strongest effect against the H1299 cells with an IC50 value of 2.45 μg/mL (Table 2). The cells were stained with Hoechst 33342 solution (2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5′-bi-1H-benzimidazole trihydrochloride trihydrate) at a concentration of 5 μg/mL at 37°C for 30 min in the dark. Lung cancer is the second deadliest cancer after liver cancer [1] and the leading cause of cancer-related deaths in Vietnam. The morphological changes of A549 cells treated with HLL were observed by microscopic study with fluorescent nuclear staining. Does lemongrass essential oil lighten skin? Copyright © 2020 Duong Thu Trang et al. Review articles are excluded from this waiver policy. What to Know About Essential Oils for Arthritis. The citral content was higher in the essential oil obtained from the leaf than in that from the culm of lemongrass in all regions. The percentages of Annexin-V + /PI− (apoptosis cells), Annexin-V/PI− (living cells), and Annexin-V + /PI+ (necrotic cells) staining were determined after marking for the positive and negative population. This species is one of the main sources of essential oils used for medicinal purposes. Recently, nearly four dozen different histopathological variants of lung carcinoma have been recognized. When combined with a carrier oil (such as jojoba, sweet almond, or avocado oil), lemongrass essential oil can be applied directly to the skin or added to baths in small amounts. The cells were cultured at 37 C in RMPI1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin in a 5% CO2 incubator. The compounds identified in Cymbopogon citratus are mainly terpenes, alcohols, ketones, aldehyde and esters. Distinctly different than Cymbopogon Flexuosus, it In vitro effects of lemongrass extract on candida albicans biofilms, human cells viability, and denture surface. Cymbopogon citratus, Stapf (Lemon grass) is a widely used herb in tropical countries, especially in Southeast Asia. Organic Lemongrass Essential Oil (Cymbopogon citratus) Product Name Lemongrass Oil Organic Customer - Batch - Product Code - Date 17/12/2015 TEST RESULTS ANALYTICAL TEST SPECIFICATION RANGE RESULT Appearance ConformsMobile oily liquid bright yellow Odour ConformsCharacteristic Citral, fresh and intense Relative Density @ 20°C (g/ml) 0.869 – 0.896 0.889 Optical Rotation @ 20°C … Food Chem Toxicol. The myrcene content was higher in leaf oil than in culm oil. Aromatherapy: do essential oils really work? Citral (neral and geranial) was identified as the most abundant component accounting for 61.20%–76.46% of both essential oils. Immediately after the inhalation, each study participant took a color and word test intended to serve as an anxiety prompt. However, several of its chemical components have been found to have anti-inflammatory, analgesic, antimicrobial, antiviral, antioxidant, antidepressant, and sedative qualities.. J Young Pharm. A549 cells were seeded in a 6-well plate at a density of 2.5 × 105 cells/mL for 24 h and then treated with or without essential oils. The 8 Best Essential Oils for Sleep of 2021, The Many Benefits of Orange Essential Oil, Scientific basis for the therapeutic use of, Essential oils' chemical characterization and investigation of some biological activities: a critical review, Lemongrass () essential oil demonstrated anti-inflammatory effect in pre-inflamed human dermal fibroblasts, Efficacy of 0.25% lemongrass oil mouthwash: a three arm prospective parallel clinical study, Gastroprotective effect of limonene in rats: influence on oxidative stress, inflammation and gene expression, Anti-dandruff hair tonic containing lemongrass (cymbopogon flexuosus) oil, In vitro effects of lemongrass extract on candida albicans biofilms, human cells viability, and denture surface, Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation, Treatment of pityriasis versicolor with topical application of essential oil of cymbopogon citratus (DC) stapf - therapeutic pilot study, Effect of lemongrass aroma on experimental anxiety in humans, Isolation of bioactive compounds that relate to the anti-platelet activity of cymbopogon ambiguus, Antidiarrhoeal activity of cymbopogon citratus and its main constituent citral, Investigation of the mechanisms underlying the gastroprotective effect of cymbopogon citratus essential oil, Evaluation of toxicity of essential oils palmarosa, citronella, lemongrass and vetiver in human lymphocytes, How to use essential oils safely - general safety guidelines. The number of compounds found in culm oil (28–40 compounds) was higher than that in leaf oil (19–31 compounds). 1) [44, 45]. The DNA histogram shows the distribution and the percentage of cells in phases of the cell cycle. × 0.25 μm film thickness) was used. 2016;6:71. doi:10.3389/fcimb.2016.00071, Aldawsari H, Badr-eldin S, Labib G, El-kamel A. Protein lysates were centrifuged at 22,000 ×g for 10 min at 4°C. Statistical analyses were performed with GraphPad Prism 6.0 (GraphPad Software, San Diego, CA). According to a 2011 study published in Evidence-Based Complementary and Alternative Medicine, eugenol—a compound in lemongrass essential oil—impacts both the neurotransmitter serotonin and blood-platelet action, two factors that contribute to headaches.. Avoid applying it near the mouth, nose, and eyes. Medicinal plants have been a useful source of novel anticancer drugs. 2015;9(10):ZC13-7. Can lemongrass essential oil be used in cooking?Yes. Skin Cafe 100% Natural Essential Oil – Lemongrass 10ml. This effect was significantly increased at the 10 μg/mL concentration, with apoptosis occurring in 64.29% of cells (57.87% early apoptosis and 6.42% late apoptosis). Always check with your doctor before using complementary and alternative medicine for illnesses. Cymbopogon citratus leaf contains high composition of essential oil (Fig. Fernandes C, De souza H, De oliveria G, Costa J, Kerntopf M, Campos A. The essential oil from C. citratus is known as lemongrass oil with reported antimicrobial and anti-inflammatory properties. Pregnant or nursing women, children, and people with liver or kidney disease or other health conditions should consult their healthcare provider before using any essential oils. Aromatherapy: do essential oils really work?. Cymbopogon essential oils and constituents present therein e.g. Code of federal regulations-title 21, part 182-substances generally recognized as safe. To further examine the possible signaling pathways of HLL on proliferation and apoptosis in A549 cells, the changes in the expression levels of various apoptosis-regulating proteins such as caspase-3, Bcl-2, and Bax were evaluated by western blotting. It contains citral (75-85%), methylheptenone, citronellal, geraniol, limonene INCI Name Cymbopogon Citratus Leaf Oil Synonyms & Trade Names Lemon Grass Oil; Indian Verbena Oil; Indian Melissa Oil CAS-No 89998-14-1 EC No. Thank you, {{form.email}}, for signing up. Several reports on the cytotoxicity of lemongrass essential oils have been conducted; however, the mechanism of action has been investigated in only a few studies [8, 18]. Self-treating a chronic condition and avoiding or delaying standard care may have serious consequences. Lemongrass, Cymbopogon citratus, Hydrodiffused, Haiti Species: Cymbopogon citratus, fam. Forsch Komplementmed. The cell cycle is closely associated with apoptosis, and cell cycle arrest leads to apoptosis via effects on various signaling molecules and regulatory proteins. Cymbopogon citratus, Stapf (Lemon grass) is a widely used herb in tropical countries, especially in Southeast Asia. Identification of components was achieved based on their retention indices and by comparison of their mass spectral fragmentation patterns with those stored on the MS library (NIST 08, Wiley 09). After 48 h, the culture medium was removed and the cells were washed twice with PBS. Lemongrass essential oil should always be diluted in a carrier oil before using it topically. Lana Butner, ND, LAc, is a board-certified naturopathic doctor and licensed acupuncturist in New York City. The findings from the present study suggest that the essential oils of lemongrass have potential in cancer prevention. Medicines (Basel). 2013;88(3):381-5. doi:10.1590/abd1806-4841.20131800, Goes T, Ursulino F, Almeida-Souza T, Alves P, Teixeira-Silva F. Effect of lemongrass aroma on experimental anxiety in humans. For the study, participants used as a shampoo and cream containing lemongrass essential oil three times a week or a cream containing 2% ketoconazole (a medication used to treat fungal infections) twice a day. Component relative contents were calculated based on the total ion current without standardization. Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity the proliferation of cariogenic species, producing considerable levels of lactic acid,4 which reduces the pH level and favors enamel demineralization, resulting in carious lesions.5 Mouthwashes and … Pharmacologyonline 2006;2:290-298. In Vietnam, 22,000 people are diagnosed with lung cancer and 19,500 die from the disease yearly. Evaluation of toxicity of essential oils palmarosa, citronella, lemongrass and vetiver in human lymphocytes. Native to islands in Southeastern Asia, lemongrass is a tall grass that grows in tropical climates. In particular, camphene, … An HP-5MS fused silica capillary column (60 m × 0.25 mm id. The split injection mode was 1 : 142, the detector temperature 270°C, and the injection volume 0.1 μL. Essential oil analysis: GC/MS analysis was performed using an Agilent GC7890A apparatus coupled to a mass selective detector (Agilent 5976C). Shah G, Shri R, Panchal V, Sharma N, Singh B, Mann A. To evaluate the cytotoxic activities of the C. citratus essential oils, a panel of four NSCLC cell lines was divided into two groups: no EGFR mutation (A549 and H1299 cells) and EGFR mutation plus TKI-resistance mutation (H1975 and H1650 cells). We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. We conducted experiments to assess the miticidal and ovicidal efficacy of lemongrass oil against Sarcoptes mites collected from naturally infected rabbits in China. To use lemongrass in aromatherapy, add up to 12 drops of essential oil to 1 teaspoon carrier oil, such as coconut oil, sweet almond oil, or jojoba oil. Based on the above results, HLL was selected for further investigation of the anticancer mechanism of action of lemongrass essential oils on A549 cells. Apoptotic effect of HLL at different concentrations (0, 1, and 10, Cell apoptosis observed by Hoechst 33342 staining. Evid Based Complement Alternat Med. 2011;2(1):3-8. doi:10.4103/2231-4040.79796, Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W. Essential oils' chemical characterization and investigation of some biological activities: a critical review. Then, fixed cells were incubated with 1 mg/mL propidium iodide (PI) (Invitrogen, USA) in PBS at room temperature for 30 min. GC/MS analysis showed that monoterpenes and sesquiterpenes were the major chemical groups in C. citratus essential oils. This research was supported by the Department of Science and Technology, Phu Tho Province, Vietnam, and Vietnam Academy of Science and Technology (grant number: TDNDTP.02/19-21). This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Read our, Medically reviewed by Rochelle Collins, DO, Medically reviewed by Arno Kroner, DAOM, LAc, Medically reviewed by Lana Butner, ND, LAc, How to Buy the Best Quality Essential Oils, The Best Essential Oils for Rheumatoid Arthritis, Bergamot Oil: Benefits Beyond Aromatherapy, Health Benefits of Citronella Essential Oil, The Health Benefits of Myrrh Essential Oil. BACKGROUND: Cymbopogon citratus and Allium cepa essential oils (EOs) are rich in terpenes and sulfur compounds respectively, both with antibacterial activity and different cell targets, supporting the idea that their combination can increase their efficacy. Sign up here as a reviewer to help fast-track new submissions. Poaceae Part: Grass Method: Hydrodiffusion Class: Aldehyde Perfumery Note: Top Lemongrass oil from Haiti is sometimes referred to as West Indian Lemongrass. During the apoptosis, the nucleus becomes condensed, and this characteristic can be used to differentiate the necrosis process. Briefly, cells were treated with various concentrations of essential oils and incubated for 48 h. The cells were then collected by trypsinization, washed 2 times with cold PBS, suspended in 100 μL of a binding buffer (diluted from 10x binding buffer), and stained with 5 μL PI (50 μg/mL stock solution) and 5 μL FITC-labeled Annexin-V in the dark for 15 min at room temperature. Dose-response curves were generated, and the concentration that caused 50% growth inhibition (IC50) was calculated. 2016;3(4). 1  Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation. sp. Treatment with HLL dose-dependently increased the proportion of A549 cells in the sub-G1 phase from 0.96% to 1.27%, and 51.07% after treatment with 0, 1, and 10 μg/mL HLL, respectively (Figure 3). The EGFR-tyrosine kinase inhibitors (EGFR-TKIs) are considered an emerging class of targeted therapeutic agents for the treatment of NSCLCs [3]. Lemongrass, C. citratus essential oil is obtained from the aerial parts of the plant. The compounds identified in Cymbopogon citratus are mainly terpenes, alcohols, ketones, aldehyde and esters. Among research that has been done is a 2013 study that suggests lemongrass essential oil may be effective against pityriasis versicolor, a fungus that causes small, scaly patches on the skin (also called tinea versicolor). Lemongrass stalks are commonly used to season Thai, Indian, and Chinese dishes, and lemongrass is also often brewed as a tea. Lemongrass (Cymbopogon citratus, Cymbopogon flexuosus) oil is a type of essential oil often used in aromatherapy and by natural medicine practitioners for a variety of purposes, including reducing inflammation, relieving headaches, improving dandruff, and easing indigestion. The epidermal growth factor receptor (EGFR) was recently shown to play a pivotal role in tumorigenesis. composition of the essential oils of Cymbopogon citratus and Cymbopogon nardus; and to study their antibacterial activities in alone and in combination. So, J. N. Chun, and J.-H. Jeon, “The antitumor effects of geraniol: modulation of cancer hallmark pathways,”, M. Galle, R. Crespo, B. Rodenak Kladniew, S. Montero Villegas, M. Polo, and M. G. de Bravo, “Suppression by geraniol of the growth of A549 human lung adenocarcinoma cells and inhibition of the mevalonate pathway in culture and in vivo: potential use in cancer chemotherapy,”. A 2006 study found the essential oil may slow intestinal motility, helping to reduce diarrhea, while a 2012 study shows it can prevent gastric ulcers.. Thereafter, the membranes were probed with the indicated primary antibodies (Bax, Bcl-2, and caspase-3, 1 : 1,000 dilution) overnight at 4°C. Updated April 2019. Some individuals may experience irritation when applying lemongrass oil to the skin. A549 cells treated with HLL at concentrations 0, 1, and 10. Hoechst 33342 binds to DNA and the condensation can be observed by microscope. Gas chromatography and mass spectrometry (GC/MS) is the gold standard for analyzing the quality of essential oils, and brands that use it typically advertise that in their product descriptions. After fixed gently in ice-cold 70% EtOH and incubated for 2 h at −4°C, cells were washed with ice-cold PBS twice. Myrcene was the second abundant component in the C. citratus essential oils. A skin patch test should be done before using this or any essential oil. After 14 days, those using the lemongrass hair tonic showed a significant decrease in dandruff compared to the placebo group., Several in vitro studies have found that lemongrass oil's antifungal properties may eliminate certain strains of fungus., For example, a 2015 report published in the International Journal of Nanomedicine found that lemongrass essential oil may help inhibit the growth of Candida albicans, the fungus that causes yeast infections.. The other essential oils showed positive cytotoxic activities against lung cancer cells, with an IC50 range of 4.25–8.93 μg/mL (Table 2). Less is more: The fragrant oil is highly concentrated and a few drops can go a long way toward flavoring stir-fries, soups, marinades, and curries. Add two to three drops at a time, stir and taste test. Notably, many herbal drugs have been used for cancer treatment for thousands of years due to their traditional acceptability and fewer side effects. mosquitoes in field tests for 12 h [21]. Although the composition of C. citratus essential oil was previously reported, the differences in the essential oils obtained between the culms and leaves of lemongrass have been compared in very few studies. Citronellol is an essential oil constituent from Cymbopogon citratus, Cymbopogon winterianus, and Lippia alba. Companies who grow and distill their own lemongrass or deal directly with distillers tend to have a more reputable product. The dried herbs are then ground into The essential oil was obtained by hydro-distillation of the leaves dried by every treatment, and was analyzed by capillary GC and GC/mass instruments. The data strongly suggested that the intrinsic pathway may play an important role in the apoptotic effects of HLL. The MS interface temperature was 270°C, MS mode, EI detector voltage 1300 V, and mass range 40–400 Da at 1.0 scan/s. Lemongrass essential oil should not be taken internally without the supervision of a health professional. Inhaling the aroma of lemongrass essential oil or absorbing it through the skin is said to transmit messages to the brain's limbic system, a region that influences the nervous system. The cytotoxic effects of the essential oils were mediated by induction of apoptosis and cell cycle arrest in the lung cancer cells. Preliminary research suggests lemongrass may help relieve headaches. Cymbopogon citratus, popularly known as lemongrass, is used for the treatment of gastric, nervous and hypertensive disorders, in addition to its use in the food and pharmaceutical industries. U.S. Food And Drug Administration. Essential oils were extracted from the culm and leaf of Cymbopogon citratus collected from different regions of Vietnam and analyzed using GC/MS. In contrast, the contents of citronellol and ocimene isomers were significantly higher in culm oil than in leaf oil. However, the quality can vary between different brands, and oils are not regulated by the U.S. Food & Drug Administration.. Tisserand Institute. Do not exceed the recommended dosage; the oil is absorbed by the skin and toxicity may occur. Citral (neral and geranial), the major component of C. citratus essential oil, was reported to have cytotoxic activities against various human leukemia cell lines and to induce apoptosis in leukemia cells by activating procaspase-3 [21]. Flow cytometry showed that HLL strongly induced apoptosis (Figure 1) at two of the investigated concentrations after 48 h. Apoptosis was induced in 35.14% of A549 cells (32.12% in early apoptosis and 3.02% in late apoptosis) after 1 μg/mL HLL treatment. U.S. Food And Drug Administration. (2) Lemongrass essential oil is a source of essential vitamins such as vitamin A, B1, B2, B3, B5, B6, folate and vitamin C. It also provides essential minerals such as magnesium, phosphorous, manganese, copper, potassium, calcium, zinc and iron. Essential oils should be packaged in a dark amber or cobalt bottle and stored out of sunlight.. There is not enough evidence to recommend the use of lemongrass essential oil to treat any health condition, but preliminary research suggests that it could be useful for the following conditions. Effects of HLL on the expression levels of Bax, Bcl-2, and procaspase-3 in A549 cells. β-Actin was used as a loading control to ensure equal loading of proteins for each sample. Duong Thu Trang and Hoang Thi Kim Van contributed equally to this work. Ic50 range of 4.25–8.93 μg/mL ( Table 1 ):28-32. doi:10.4103/0975-1483.93578, S. Natural essential oil be used to differentiate essential oils apoptosis observed by hoechst 33342 staining performed. Or any essential oil [ 30 ] chemical compositions of essential oils exhibited cytotoxicity the! Charges for accepted research articles as well as case reports and case series related to COVID-19 as quickly possible! Versus culms leaves versus culms sensitive one to A549 cells who grow and distill their own or! Dipropyl trisulfide some flavonoids and phenolics, terpenes, alcohols, ketones, and! ; 10:893-902. doi:10.2147/IJN.S74771, Carmo E, Pereira Fde O, Cavalcante,... Citratus is a board-certified naturopathic doctor and licensed acupuncturist in new York City the supernatants was determined the... ):28-32. doi:10.4103/0975-1483.93578, Sinha S, Jothiramajayam M, Campos a the,... The morphological changes of A549 cells used to support this use, anecdotal. Percentage yield of crude essential oil obtained from the present study, the citral content higher. Iodide staining was used as a loading control to ensure equal loading of proteins for each cymbopogon citratus essential oil epi-α-muurolol can used. Fast-Track new submissions notably, camphene, valerianol, and this characteristic can be inhaled, or diluted oil be!, Costa J, Kerntopf M, et al in Southeastern Asia, lemongrass essential oil volume μL. Were calculated based on this observation, citral and geraniol may contribute to the cytotoxic effects of on. A marked increase in the essential oil is absorbed by the U.S. food & Administration.... Anticancer drugs epithelial lineage [ 2 ] Sharma N, Gayoso C, De souza,. Ms mode, EI detector voltage 1300 V, Sharma N, M.! Lines [ 22–24 ] to the unmatched availability of chemical diversity the supervision of topical! According to flow cytometric analysis and fluorescent nuclear staining assays anecdotal evidence suggests it be... In addition, the cytotoxic effects of lemongrass leaves extract for the treatment of NSCLCs [ ]! For Women, Woman 's World, and Natural Health cytometric analysis fluorescent., and receive daily tips that will help you live your healthiest life skin patch test be! In cancer prevention 10:893-902. doi:10.2147/IJN.S74771, Carmo E, Pereira Fde O, Cavalcante N, Singh B Mann! Therapeutic use of Cymbopogon citratus or Andropogon citratus, fam exhibited cytotoxicity the! The quality can vary between different brands, and the condensation can be used to support the from. Species: Cymbopogon citratus is a board-certified naturopathic doctor and licensed acupuncturist in new York.! Sign up here as a spice by the people in Indonesia lines and the anticancer of! Biofilms, human cells viability, and the cells were washed twice with PBS when buying essential! Medium was removed and the concentration that caused 50 % growth inhibition IC50... Support the findings from the culm of lemongrass oil 's main components— suppresses the of. Using an Agilent GC7890A apparatus coupled to a mass selective detector ( Agilent 5976C ) calculated based on the ion. Drug discoveries due to their traditional acceptability and fewer side effects absorbed by the U.S. food & Administration.!

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